Abacwaningi basungule inqubo ye-biotechnology eguqula imfucuza yepulasitiki ye-polyethylene terephthalate (PET) ibe yi-levodopa (L-DOPA) – umuthi oyinhloko wokwelapha isifo sikaParkinson. Le ndlela isebenzisa i-Escherichia coli eguqulwe izakhi zofuzo futhi ithola isivuno esikhulu ngaphansi kwezimo ezithambile nezimanzi.
Ithimba eliholwa nguBenjamin Royer noStephen Wallace lakha indlela ye-biosynthetic enezinyathelo ezine eguqula i-terephthalic acid (TPA) – i-monomer eyinhloko ye-PET – ibe yi-L-DOPA nge-protocatechuate kanye ne-catechol. Izingqinamba ezimbili ezibalulekile – ukungenisa kwe-substrate kanye nokuvinjelwa kwempendulo yi-protocatechuate – zanqotshwa ngokuveza i-TPA transporter (TpaK) kusuka ku-Rhodococcus jostii nokuhlukanisa indlela ibe izinhlobo ezimbili (eyodwa ye-TPA kuya ku-catechol, enye ye-catechol kuya ku-L-DOPA).
Enqubweni eyenziwe kahle yokuxuba izinhlobo ezimbili, kutholakale u-5,0 g/l we-L-DOPA ku-5 mM TPA. Le nqubo yasetshenziswa ngempumelelo kudoti we-PET wezimboni kanye nebhodlela elilodwa lepulasitiki elisetshenzisiwe; amanani okulungiselela kasawoti we-L-DOPA ahlukaniswa.
Ukuze kuthuthukiswe ukusimama, i-CO₂ ekhishwe ekususweni kwe-TPA yathathwa yi-microalga Chlamydomonas reinhardtii – ubufakazi bomqondo wokusetshenziswa kabusha kwe-CO₂ kule nqubo.
Lo msebenzi ubonisa indlela i-biology yobunjiniyela engaguqula ngayo imfucuza yepulasitiki ibe yimithi yemithi ebiza kakhulu futhi inikeza enye indlela yokwenziwa kwezindlela ezisekelwe ezintweni zasendulo ze-L-DOPA. Ukukhiqizwa kwamanje (~250 t/ngonyaka) kusekelwe ezindleleni ze-petrochemical noma ze-chemoenzymatic, ezibhekene nezinselele mayelana nokusebenza kahle kwekhabhoni kanye nokusimama.
umthombo:
Ukusimama Kwemvelo, “Ukuvuselelwa kabusha kwemfucuza yepulasitiki ibe yi-levodopa ngama-microbial”, nguBenjamin Royer, Yuta Era et al. (uStephen Wallace njengombhali ohambisanayo), eyanyatheliswa ngoMashi 16, 2026
I-DOI: 10.1038/s41893-026-01514-0 (Ukufinyelela Okuvulekile)
https://www.nature.com/articles/s41893-026-01514-0
